FRACTIONAL ELECTRICAL TRANSPORT STUDIES OF VITAMIN B12 BINDING BY PROTEINS
نویسندگان
چکیده
منابع مشابه
Fractional electrical transport studies of vitamin B12 binding by proteins.
Bird and Hoevet (1) have described a procedure for measuring the vitamin Blz-binding capacity of protein mixtures derived from hog mucosa, based on the non-dialyzability of the protein-bound vitamin. They also observed that lysozyme would bind vitamin B1, as previously reported (3), but that it did not appear to retain the vitamin when dialyzed. However, the diffusibility of lysozyme through ce...
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Ternberg and Eakin (1) showed that normal gastric juice or an aqueous extract of hog mucosa when added unheated to vitamin Blz rendered the vitamin non-dializable and unavailable to assay microorganisms, including Lactobacillus leichmannii. They concluded that this principle which combined with vitamin Blz was the intrinsic factor of Castle or an important component thereof. Meyer et al. (2) no...
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The chromosomal location of two genetic loci involved in the transport of cyanocobalamin (B(12)) in Escherichia coli K-12 was determined. One gene, btuA, is believed to code for the transport protein in the cytoplasmic membrane, because a mutant with an alteration in this gene has lost the ability to accumulate B(12) within the cell although normal levels of the surface receptors for B(12) are ...
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A reliable sensitive method for measuring vitamin B12 in the circulation was first achieved at Hammersmith in 1950.' During the subsequent decade the vitamin B12 status was assessed of patients with a wide variety of conditions.2 This culminated in the determination of a unique function for the ileum by Booth and Mollin: that of the absorption of vitamin B123. During the course of these early s...
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Serum cobalamin (vitamin B12) and unsaturated B12 binding capacity (UBBC) have been measured in 24 cases of hypereosinophilia: 16 were cases of hypereosinophilic syndrome (HES) and 8 of secondary eosinophilia. The two groups were similar with respect to absolute eosinophil counts. Serum cobalamin and UBBC were found to be markedly increased in most cases of HES and normal in secondary eosinophi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)66198-8